| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Bowling Green High School | A | Griffith | Mix | Finnegan | Matlock | 8:37.54 | Verified |
| 2 | Mark Twain High School | A | Miller | Black | McLeod | Northcutt | 8:42.36 | Verified |
| 3 | Elsberry High School | A | Dodson | Heras | Santos | Byers | 8:52.08 | Verified |
| 4 | Pittsfield | A | Oitker | Cooper | Olson | Veile | 8:59.68 | Verified |
| 5 | Monroe City High School | A | Lehenbauer | Hunter | Richardson | Lorenson | 9:21.16 | Verified |
| 6 | Wellsville-Middletown High Schoo | A | Bickell | Marshall | Slovensky | Vogel | 9:50.73 | Verified |
| 7 | Louisiana High School | A | Martin | Hathaway | Pitzer | Silvey | NT | Unverified: |