| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | JOHNSON, Taylor | JR | Community High School | 36.56 | Verified |
| 3 | SIMMONS, Sophie | FR | Clopton High School | 33.56 | Verified |
| 4 | HAYS, Addyson | FR | Van-Far High School | 34.78 | Verified |
| 5 | JOHNSON, Chloe | FR | Community High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | SLATER, Sophia | FR | Canton High School | 31.83 | Verified |
| 2 | INCE, Lauren | SO | Louisiana High School | 31.65 | Verified |
| 3 | RILEY, Kamryn | FR | Pittsfield | 31.11 | Verified |
| 4 | BOECKELMAN, Natalie | SR | Wellsville-Middletown High Schoo | 31.60 | Verified |
| 5 | MARTIN, Mya | FR | Monroe City High School | 31.65 | Verified |
| 6 | GAY, Dalana | SO | Van-Far High School | 33.21 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | JOHNSON, Ava | FR | Bowling Green High School | 30.33 | Verified |
| 2 | PREIST, Rynleigh | SO | Pittsfield | 29.90 | Unverified: |
| 3 | CHARLTON, Kaylyn | JR | Bowling Green High School | 29.06 | Verified |
| 4 | TALBERT, Stacia | JR | Clopton High School | 29.30 | Verified |
| 5 | ROSS, Audrey | SR | Mark Twain High School | 30.20 | Verified |
| 6 | CLARK, Jaelynn | JR | Monroe City High School | 30.47 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | TALBOT, Joanna | SO | Elsberry High School | 28.67 | Verified |
| 2 | CLAY, Nariah | SR | Canton High School | 28.15 | Verified |
| 3 | SLOVENSKY, Bethany | JR | Wellsville-Middletown High Schoo | 27.91 | Verified |
| 4 | PEDERSEN, Jordan | SO | Louisiana High School | 28.01 | Verified |
| 5 | BOLEACH, Madison | JR | Mark Twain High School | 28.17 | Verified |
| 6 | IRVING, Audrianna | JR | Elsberry High School | 28.85 | Verified |