| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CLAY, Nariah | SR | Canton High School | 2:23.70 | Verified |
| 2 | HARTLEY, Ellie | FR | Elsberry High School | 2:31.31 | Verified |
| 3 | WILLIAMS, Hannah | SO | Pittsfield | 2:38.00 | Unverified: |
| 4 | MILLER, Sydney | SO | Mark Twain High School | 2:43.16 | Verified |
| 5 | FORMAN, Brooklyn | JR | Van-Far High School | 2:45.99 | Verified |
| 6 | SHELTON, Abigail | SO | Van-Far High School | 2:51.06 | Verified |
| 7 | HAYS, Ella | JR | Monroe City High School | 2:52.39 | Verified |
| 8 | TOELKE, Katelyn | SO | Pittsfield | 2:57.06 | Verified |
| 9 | BIBB, Dianna | SO | Bowling Green High School | 2:57.71 | Verified |
| 10 | ROBERTS, Ava | FR | Mark Twain High School | 3:00.15 | Verified |
| 11 | TREDWAY, Ashlynn | JR | Louisiana High School | 3:00.71 | Verified |
| 12 | TRUETKEN, Katherine | FR | Clopton High School | 3:05.90 | Verified |
| 13 | EGGEMEYER, Victoria | JR | Clopton High School | 3:16.85 | Verified |
| 14 | HAYS, Marissa | FR | Monroe City High School | 3:19.95 | Verified |
| 15 | MCCURDY, Amy | SO | Community High School | 3:43.48 | Verified |
| 16 | WELLS, Autumn | FR | Bowling Green High School | NT | Unverified: |