| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HICKS, Sophie | FR | Mark Twain High School | NT | Unverified: |
| 2 | NORVELL, Harley | JR | Louisiana High School | 58.27 | Verified |
| 3 | BANKHEAD, Ava | FR | Clopton High School | 57.55 | Verified |
| 4 | CHARLTON, Sydney | SO | Bowling Green High School | 57.97 | Verified |
| 5 | JOHNSON, Taylor | JR | Community High School | 1:28.78 | Verified |
| 6 | SPERRY, Kendra | SO | Louisiana High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | FOSS, Gracee | SO | Monroe City High School | 53.40 | Verified |
| 2 | TEN EYCK, Ellie | SR | Pittsfield | 50.47 | Unverified: |
| 3 | YOUNGBLOOD, Audri | SO | Monroe City High School | 48.05 | Verified |
| 4 | SLOVENSKY, Bethany | JR | Wellsville-Middletown High Schoo | 48.65 | Verified |
| 5 | DISALVO, Victoria | SR | Community High School | 51.43 | Verified |
| 6 | MYERS, Faith | SR | Clopton High School | 56.58 | Verified |