| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Wellsville-Middletown High Schoo | A | Boeckelman | Boeckelman | Scott | Stroup | 1:00.92 | Verified |
| 3 | Clopton High School | A | Palmer | Talbert | Bankhead | Williamson | 59.44 | Verified |
| 4 | Van-Far High School | A | Engh-Hoffman | Brooksher | Gay | Shelton | 1:00.67 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Monroe City High School | A | Foss | Martin | Clark | Hays | 57.11 | Verified |
| 2 | Mark Twain High School | A | Boleach | Hudelson | Hawn | Ross | 55.24 | Verified |
| 3 | Bowling Green High School | A | Dean | Johnson | Deters | Charlton | 53.55 | Verified |
| 4 | Louisiana High School | A | Campbell | Ries | Chatman | Wallace | 53.81 | Verified |
| 5 | Canton High School | A | Hudnut | Ott | McReynolds | Clay | 56.46 | Verified |