| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | St. James Middle School | A | Byfield | Giacalone | Jones | Hanson | NT | Unverified: |
| 3 | West County MS | A | Elder | Govreau | Gray | Twomey | 2:16.51 | Verified |
| 4 | Cuba Middle School | A | Barnwell | Hart | Munoz | Wheeler | 2:19.14 | Verified |
| 5 | Steelville Middle School | A | Enloe | Harmon | Kennedy | Richter | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | St. Clair Junior High School | A | Roemer | McDaniel | Morrison | Hallum | 1:51.95 | Verified |
| 3 | Newburg Middle School | A | Dyer | Steffe | Allen | Weible | 1:50.84 | Unverified: |
| 4 | Salem MS | A | Harris | Cunningham | Head | Connors | 1:51.47 | Unverified: |
| 5 | Rolla MS | A | Balch | Steen | Westover | Wilkins | 1:52.99 | Verified |
| 6 | Vienna Middle School | A | Crandle | Hallahan | Summerford | Ewers | 2:03.28 | Verified |