| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Salem MS | A | Howard | Terell | Wilson | Chambers | NT | Unverified: |
| 3 | Steelville Middle School | A | Skinner | Adams | West | Worley | 2:19.80 | Verified |
| 4 | Cuba Middle School | A | Bright | Knutson | Lorenz | Farris | 2:23.72 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Vienna Middle School | A | Campbell | Gorman | Schwartze | Smith | 2:11.84 | Verified |
| 2 | St. Clair Junior High School | A | Ochsenbine | Conner | Dykhuizen | Martin | 2:07.50 | Verified |
| 3 | Rolla MS | A | Armstrong | Delagarza | Northup | Register | 2:03.92 | Verified |
| 4 | St. James Middle School | A | Vass | Davis | Snelson | Parsons | 2:04.23 | Unverified: |
| 5 | Crocker Middle School | A | Ament | Danielson | Medlen | Sharpensteen | 2:10.96 | Verified |
| 6 | Newburg Middle School | A | Caquelin | Nelson | Sherrer | Steffe | 2:16.00 | Unverified: |