| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Steelville Middle School | A | Smelser | Turnbough | Settles | Hurd | 13:00.68 | Verified |
| 2 | Rolla MS | A | Harrison | Harrison | Rivera | Wilson | 13:04.66 | Verified |
| 3 | Vienna Middle School | A | Carpenter | Hawk | McKinnon | Neubert | 13:30.28 | Verified |