| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Salem MS | A | Ray | Cunningham | Edmondson | Moody | 4:36.25 | Unverified: |
| 2 | St. Clair Junior High School | A | Hallum | Morrison | Benkert | Richards | 4:26.47 | Verified |
| 3 | Rolla MS | A | Fox | Duehning | Fischer | Baxter | 4:09.22 | Verified |
| 4 | St. James Middle School | A | Spurgeon | Jones | Holt | Hanson | 4:20.00 | Unverified: |
| 5 | Vienna Middle School | A | Reeves | Timm | Ewers | Wiles | 4:28.78 | Verified |
| 6 | Newburg Middle School | A | Allen | Millburg | Weible | Millburg | 5:04.00 | Unverified: |
| 7 | Cuba Middle School | A | Brown | Munoz | Emery | Hart | 4:54.69 | Verified |