| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Cuba Middle School | A | Villemure | Poynter | Dickens | Smith | 5:58.46 | Verified |
| 2 | Newburg Middle School | A | Caquelin | Nelson | Sherrer | Steffe | 5:28.00 | Unverified: |
| 4 | St. Clair Junior High School | A | Conner | Martin | Ochsenbine | Hinson | 4:52.04 | Verified |
| 5 | Steelville Middle School | A | Hurd | Smelser | Schmidt | Perkins | 5:07.86 | Verified |
| 6 | Rolla MS | A | Armstrong | Kuchar | Montgomery | Register | 5:23.90 | Verified |
| 7 | Vienna Middle School | A | Bresnahan | Campbell | Magner | Smith | 5:31.98 | Verified |
| 8 | Salem MS | A | Green | Johnson | Schlag | Wallace | NT | Unverified: |