| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Vienna Middle School | A | Hatfield | Hallahan | Murray | LeStarge | 1:01.35 | Verified |
| 3 | West County MS | A | Simily | Gray | Miller | Carroll | 58.06 | Verified |
| 4 | Steelville Middle School | A | Enloe | Harmon | Bell | Signaigo | 58.75 | Verified |
| 5 | St. James Middle School | A | Gipson | Weinbaum | Ridenhour | Hanson | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Cuba Middle School | A | DeLuna | Wheeler | Shireman | Fey | 54.68 | Verified |
| 2 | Salem MS | A | Harris | Land | Head | Ray | 52.52 | Unverified: |
| 3 | Rolla MS | A | Balch | Steen | Westover | Wilkins | 49.53 | Verified |
| 4 | Newburg Middle School | A | Allen | Killian | Dyer | Brookshire | 51.95 | Unverified: |
| 5 | St. Clair Junior High School | A | Roemer | Morrison | McDaniel | Wright | 52.85 | Verified |
| 6 | Crocker Middle School | A | Carriger | Devine | Harrison | Sherrell | NT | Verified |