| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Cuba Middle School | A | Evard | Humphrey | Schmidt | Garbo | NT | Unverified: |
| 2 | West County MS | A | Dicus | McGee | Hurd | Wring | 1:09.02 | Verified |
| 3 | Steelville Middle School | A | Worley | Adams | Brinkley | Kreitner | 1:04.41 | Verified |
| 4 | Newburg Middle School | A | Caquelin | Nelson | Parks | Steffe | 1:05.00 | Unverified: |
| 5 | Salem MS | A | Wilson | Beyer | Chambers | Connell | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Stoutland Middle School | A | Cornelison | Franklin | Howlett | Miller | 1:01.23 | Unverified: |
| 2 | St. Clair Junior High School | A | Ochsenbine | Martin | Johnson | Conner | 58.54 | Verified |
| 3 | Rolla MS | A | Armstrong | Delagarza | Kuchar | Northup | 57.71 | Verified |
| 4 | St. James Middle School | A | Davis | Roark | Richardson | Parsons | 58.23 | Unverified: |
| 5 | Crocker Middle School | A | Sharpensteen | Ament | Stewart | Danielson | 1:00.46 | Verified |
| 6 | Vienna Middle School | A | Magner | Hollis | Smith | Schwartze | 1:04.18 | Verified |