| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Bland Middle School | A | Frey | Gromowski | Martin | LeRoux | 2:11.70 | Verified |
| 4 | Steelville Middle School | A | Harmon | Kennedy | Signaigo | West | 2:04.97 | Verified |
| 5 | Bourbon Middle School | A | Troutt | King | Lappe | Sanders | 2:10.73 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Newburg Middle School | A | Dyer | Gates | Killian | Steffe | 1:49.95 | Verified |
| 2 | Sullivan Middle School | A | Marler | Ray | Redd | Breen | 1:47.40 | Verified |
| 3 | St. Clair Junior High School | A | Hill | Perkins | Roemer | Morrison | 1:46.56 | Verified |
| 4 | Waynesville Middle School | A | Fau | Rinck | Perry | Gonzales | NT | Verified |
| 5 | Union Middle School | A | Glenville | Cowan | Webb | Kennedy | 1:44.78 | Verified |
| 6 | St. James Middle School | A | Rector | Freeman | Hanson | Holt | 1:46.72 | Verified |
| 7 | Cuba Middle School | A | Lewis | Hart | Brown | Shireman | 1:49.90 | Verified |
| 8 | Vienna Middle School | A | Kleffner | Wiles | Ewers | Hatfield | 2:03.28 | Verified |