| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Bourbon Middle School | A | Cole | Cunningham | Sanders | Waldron | 2:15.19 | Verified |
| 4 | St. Paul Lutheran MS (Farmington | A | Daniel | Keddy | Petty | Crites | 2:12.19 | Verified |
| 5 | Newburg Middle School | A | Caquelin | Nelson | Sherrer | Steffe | 2:14.17 | Verified |
| 6 | Cuba Middle School | A | Bright | Knutson | Lorenz | Basham | 2:19.06 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Steelville Middle School | A | Worley | Skinner | Adams | Perkins | 2:09.95 | Unverified: |
| 2 | Bland Middle School | A | Prater | Rhoads | Rhoads | Smith | 2:02.58 | Verified |
| 3 | Waynesville Middle School | A | Wheat | Doig | Pelliccia | McClure | 2:00.85 | Verified |
| 4 | St. Clair Junior High School | A | Hinson | Conner | Ochsenbine | Webb | 1:58.21 | Verified |
| 5 | Sullivan Middle School | A | Harbour | Pohlman | Martin | Schmuke | 2:00.76 | Verified |
| 6 | St. James Middle School | A | Vass | Davis | Snelson | Parsons | 2:02.28 | Verified |
| 7 | Union Middle School | A | Colombo | Brown | Weber | Prichard | 2:02.59 | Verified |
| 8 | Vienna Middle School | A | Schwartze | Gorman | Smith | Magner | 2:11.84 | Verified |