| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Bourbon Middle School | A | Wallis | Froning | Dudley | Sanders | 5:17.06 | Verified |
| 4 | Vienna Middle School | A | Wiles | Reeves | Kleffner | Ewers | 4:28.78 | Verified |
| 5 | Union Middle School | A | Beckmann | Richoz | Gillespie | Prilwetz | 4:30.14 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Cuba Middle School | A | Brown | Hart | Lewis | DeLuna | 4:24.16 | Verified |
| 2 | Sullivan Middle School | A | Gassner | Duncan | Marler | Westbrook | 4:19.88 | Verified |
| 3 | St. Clair Junior High School | A | Benkert | Hill | Perkins | Morrison | 4:15.71 | Verified |
| 4 | Waynesville Middle School | A | MacMurray | Rinck | Hyde | Hightower Henton | 3:50.95 | Verified |
| 5 | St. James Middle School | A | Smith | Freeman | Beam | Bell | 4:01.53 | Verified |
| 6 | Bland Middle School | A | Robertson | Fredendall | Skaggs | Streumph | 4:19.59 | Verified |
| 7 | Newburg Middle School | A | Dyer | Allen | Steffe | Weible | 4:21.97 | Verified |