| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Bourbon Middle School | A | Deschada | Ham | Waldron | Rohrer | 5:41.55 | Verified |
| 4 | Vienna Middle School | A | Smith | Schwartze | Gorman | Magner | 5:23.49 | Verified |
| 5 | Cuba Middle School | A | Dickens | Villemure | Thruman | Poynter | 5:37.09 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Steelville Middle School | A | Schmidt | Turnbough | Smelser | Perkins | 4:54.12 | Verified |
| 2 | St. Clair Junior High School | A | Hinson | Conner | Martin | Ochsenbine | 4:48.10 | Verified |
| 3 | St. James Middle School | A | Kemnitzer | Parsons | Richardson | Roark | 4:37.06 | Verified |
| 4 | Waynesville Middle School | A | Madison | Pelliccia | Lercher | Fils | 4:25.10 | Verified |
| 5 | Sullivan Middle School | A | Lambert | Harbour | Denney | Denney | 4:33.26 | Verified |
| 6 | Union Middle School | A | Sulschi | Weber | Kuelker | Brown | 4:43.15 | Verified |
| 7 | Bland Middle School | A | Prater | Rhoads | Bray | Rhoads | 4:52.76 | Verified |
| 8 | Newburg Middle School | A | Caquelin | Nelson | Sherrer | Steffe | 5:17.10 | Verified |