| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Newburg High School | A | Caquelin | Fite | Rosa | Twyman | NT | Unverified: |
| 4 | Owensville High School | A | Gehlert | Limberg | Daniels | Daniels | 5:04.00 | Verified |
| 5 | Cuba High School | A | Black | Ijames | Weber | Wools | 5:37.62 | Verified |