| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | ATKINSON, Braden | JR | Salem High School | 2:04.95 | Verified |
| 2 | GREEN, Gavyn | SO | Salem High School | 2:13.24 | Verified |
| 3 | PAYNE, Brett | FR | Bourbon High School | 2:13.37 | Verified |
| 4 | CROCKER, Vincent | SO | Steelville High School | 2:16.77 | Verified |
| 5 | RADFORD, Brayden | SO | Salem High School | 2:26.43 | Verified |
| 6 | BAILEY, Curtis | JR | Steelville High School | 2:28.64 | Verified |
| 7 | GOLLAHER, Marshal | JR | Salem High School | 2:30.22 | Verified |
| 8 | HARRIS, Tyson | JR | Steelville High School | 2:41.14 | Verified |
| 9 | SAPPINGTON, Elijah | JR | Bourbon High School | 2:48.17 | Verified |
| 10 | DECKER, Alex | JR | Newburg High School | NT | Unverified: |
| 11 | JONES, Max | FR | Cuba High School | NT | Unverified: |
| 12 | TURNER, Zeke | SR | Newburg High School | NT | Unverified: |
| 13 | LEWIS, James | FR | Salem High School | NT | Unverified: |