| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | RADFORD, Brayden | SO | Salem High School | NT | Unverified: |
| 4 | WELCH, Jonathon | FR | Salem High School | NT | Unverified: |
| 5 | TAUSCHER, Marshall | FR | Viburnum High School | NT | Unverified: |
| 6 | JONES, Max | FR | Cuba High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MORROW, Evin | FR | Newburg High School | NT | Unverified: |
| 2 | LEWIS, James | FR | Salem High School | NT | Unverified: |
| 3 | SETZER, Boston | SO | Steelville High School | 1:06.15 | Verified |
| 4 | MEDLOCK, Eli | SR | Viburnum High School | 1:00.74 | Verified |
| 5 | HOLTMEYER, Noah | SR | Owensville High School | 1:03.30 | Verified |
| 6 | BOLLMAN, Kyle | SO | Owensville High School | 1:09.19 | Verified |
| 7 | MCDANIEL, Sean | FR | Salem High School | NT | Unverified: |
| 8 | MARTIN, Alex | FR | Steelville High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | PIETRASCHKE, Nicholas | JR | Bourbon High School | 58.32 | Verified |
| 2 | GUERRERO, Felix | JR | Owensville High School | 56.50 | Unverified: |
| 3 | ATKINSON, Braden | JR | Salem High School | 55.53 | Verified |
| 4 | DIAZ, Conner | SR | Steelville High School | 53.12 | Verified |
| 5 | CAMPBELL, Wyatt | JR | Salem High School | 54.62 | Verified |
| 6 | GREEN, Gavyn | SO | Salem High School | 55.72 | Verified |
| 7 | REEVES, Zackary | JR | Bourbon High School | 56.72 | Verified |
| 8 | BAYLESS, Xavier | SO | Owensville High School | 1:00.54 | Verified |