| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | KING, Kaitlyn | FR | Bourbon High School | NT | Unverified: |
| 2 | PRATT, Ciarra | JR | Newburg High School | 1:18.95 | Verified |
| 3 | COLLINSON, Aleah | JR | Steelville High School | 1:16.27 | Verified |
| 4 | KANDLBINDER, Lauren | SO | Owensville High School | 1:14.94 | Verified |
| 5 | LOGAN, Caitlin | SO | Newburg High School | 1:15.75 | Verified |
| 6 | BUSSELL, Allyson | SR | Cuba High School | 1:18.85 | Verified |
| 7 | WEBER, Bailey | FR | Cuba High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | IJAMES, Izzabelle | JR | Cuba High School | 1:12.15 | Verified |
| 2 | CONAWAY, Jorja | JR | Viburnum High School | 1:10.88 | Verified |
| 3 | LIMBERG, Ilene | SO | Owensville High School | 1:07.89 | Verified |
| 4 | PRYOR, Daylan | SR | Steelville High School | 1:05.14 | Verified |
| 5 | ENKE, Marisa | SO | Bourbon High School | 1:06.71 | Verified |
| 6 | CLONTS, Cierra | JR | Bourbon High School | 1:09.78 | Verified |
| 7 | WRIGHT, Brynn | SO | Owensville High School | 1:10.94 | Verified |
| 8 | WOOLS, Kailey | SO | Cuba High School | 1:14.28 | Verified |