| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Salem High School | A | Campbell | Mendenhall | Owen | Vernellus | NT | Unverified: |
| 4 | Cuba High School | A | Volner | Smith | Halbert | Dake | 48.63 | Verified |
| 5 | Owensville High School | A | Elliott | Evans | Meyer | Whelan | 49.88 | Verified |
| 6 | Steelville High School | A | Brown | Midyett | Killeen | Harris | NT | Unverified: |