| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Salem High School | A | Manthey | Pipp | Kiser | Owen | NT | Unverified: |
| 4 | Owensville High School | A | Richardson | Nowack | Echols | Daniels | 56.06 | Verified |
| 5 | Cuba High School | A | Lunsford | Dunham | Moreland | Viramontes | 1:01.84 | Verified |