| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | PAYNE, Brett | FR | Bourbon High School | 4:45.76 | Verified |
| 2 | CROCKER, Vincent | SO | Steelville High School | 5:06.68 | Verified |
| 3 | DIEHL, Alexander | SO | Salem High School | 5:27.06 | Verified |
| 4 | PARKS, Matthew | SO | Salem High School | 5:28.27 | Verified |
| 5 | GOLLAHER, Marshal | JR | Salem High School | 5:33.00 | Verified |
| 6 | MORELAND, Austin | JR | Steelville High School | 5:42.01 | Verified |
| 7 | BROOKS, Gabriel | JR | Viburnum High School | 5:42.51 | Verified |
| 8 | BAILEY, Curtis | JR | Steelville High School | 5:43.58 | Verified |
| 9 | BIRDSONG, Eli | SR | Newburg High School | 6:02.31 | Verified |
| 10 | HARRIS, Tyson | JR | Steelville High School | 6:26.19 | Verified |
| 11 | DECKER, Alex | JR | Newburg High School | NT | Unverified: |
| 12 | BROWN, Brendan | FR | Salem High School | NT | Unverified: |
| 13 | THIES, Nicholas | FR | Salem High School | NT | Unverified: |
| 14 | SHERMAN, Jordin | FR | Salem High School | NT | Unverified: |
| 15 | RUTLEDGE, Landon | FR | Steelville High School | NT | Unverified: |
| 16 | DEHURT, Kurtis | JR | Salem High School | NT | Unverified: |