| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | PERONA, Vanessa | JR | St. James High School | 5:57.31 | Verified |
| 2 | TURNBOUGH, Audrey | JR | Steelville High School | 5:58.25 | Verified |
| 3 | BERKEREY, Josie | FR | St. Paul Lutheran (Farmington) | 6:10.85 | Verified |
| 5 | MCDANIEL, Tajel | SO | Belle High School | 6:21.94 | Verified |
| 6 | CRONIN, Janelle | SO | New Haven High School | 6:23.16 | Verified |
| 7 | FITE, Brooklyn | FR | Newburg High School | 6:26.17 | Verified |
| 8 | BASHAM, Cadynce | FR | Cuba High School | 6:42.25 | Verified |
| 9 | GRUBB, Sydney | JR | New Haven High School | 6:43.78 | Verified |
| 10 | HARDCASTLE, Patricia | FR | Cuba High School | 6:51.33 | Verified |
| 11 | POHLSANDER, Alexandria | FR | Rolla High School | 7:03.46 | Verified |
| 12 | COLLINSON, Lydia | FR | Steelville High School | 7:08.84 | Verified |
| 13 | RANGEL, Samaya | FR | Dixon High School | 7:46.21 | Verified |
| 14 | JO WILSON, Andy | FR | Dixon High School | NT | Unverified: |