| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | St. Paul Lutheran (Farmington) | A | Folk | Folk | Jordan | Anderson | 1:59.39 | Verified |
| 4 | Vienna High School | A | Baz | Douglas | Kilmer | Carnes | 1:45.44 | Verified |
| 5 | Belle High School | A | Weigel | Gromowski | Valley | Smith | 1:51.77 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Dixon High School | A | Dalton | Hurst | Rester | O'neal | 1:45.15 | Verified |
| 3 | Cuba High School | A | DeClue | West | Bonney | Smith | 1:41.46 | Verified |
| 4 | Rolla High School | A | Plank | Harris | Wassilak | Gibson | 1:36.46 | Verified |
| 5 | Steelville High School | A | Brown | Killeen | Midyett | Settles | 1:38.02 | Verified |
| 6 | St. James High School | A | Bullock | Earls | Noakes | Hancock | 1:43.62 | Verified |
| 7 | Crocker High School | A | Bjornstead | Goldsmith | Faulkner | Sharpensteen | 1:45.42 | Verified |