| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | St. Paul Lutheran (Farmington) | A | Constien | Govero | Durbin | Klima | 2:21.56 | Verified |
| 4 | Cuba High School | A | West | Weber | Viramontes | Black | 2:08.65 | Verified |
| 5 | Newburg High School | A | Brinkley | Logan | Pratt | Rosa | 2:16.93 | Verified |
| 6 | Missouri School for the Deaf | A | Davis | Austin | Hull | Smith | 2:26.14 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Bourbon High School | A | Clonts | King | Poole | Enke | 2:01.21 | Unverified: |
| 3 | Vienna High School | A | Novak | Reeves | Schell | Schulte | 2:00.28 | Verified |
| 4 | Rolla High School | A | Inskip | Alamaldein | Brooks | Kriete | 1:55.67 | Verified |
| 5 | St. James High School | A | Freeman | Deardeuff | Holt | Campbell | 1:57.10 | Unverified: Union Invitational |
| 6 | Steelville High School | A | Kelly | Diaz | Pryor | Pryor | 2:00.44 | Verified |
| 7 | New Haven High School | A | Meyer | GUEHNE | FLEER | Groner | 2:02.24 | Verified |
| 8 | Viburnum High School | A | Pyatt | Halbert | Conaway | Wigger | 2:07.96 | Verified |