| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | COLON, Izabella | SO | Bourbon High School | NT | Unverified: |
| 4 | MAUNTEL, Kyra | JR | New Haven High School | 21.81 | Verified |
| 5 | BEDFORD, Brynley | FR | Rolla High School | 21.90 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | KOCH, Olivia | SR | Belle High School | 21.37 | Verified |
| 2 | BARTLE, Raylee | SO | Bourbon High School | 20.63 | Verified |
| 3 | MARTELLARO, Rachel | SO | Cuba High School | 20.19 | Verified |
| 5 | HALBERT, Natalie | SO | Viburnum High School | 20.14 | Verified |
| 6 | NOVAK, Madeleine | SR | Vienna High School | 20.57 | Verified |
| 7 | TONEY, Addison | FR | Rolla High School | 21.27 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | EILERT, Marley | FR | Steelville High School | 19.24 | Verified |
| 2 | SNYDER, Ava | FR | St. James High School | 19.06 | Verified |
| 3 | TWYMAN, Corra | SR | Newburg High School | 18.28 | Verified |
| 4 | KECK, Montgomery | JR | Dixon High School | 15.28 | Verified |
| 5 | KREITNER, Abby | JR | Steelville High School | 18.19 | Verified |
| 6 | KLIMA, Carley | FR | St. Paul Lutheran (Farmington) | 18.69 | Verified |
| 7 | CONSTIEN, Sarah | SO | St. Paul Lutheran (Farmington) | 19.08 | Verified |
| 8 | DAVIS, Julianna | FR | Vienna High School | 19.50 | Unverified: |