| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | St. Paul Lutheran (Farmington) | A | Peak | Anderson | Prade | Jordan | 4:17.36 | Verified |
| 2 | Cuba High School | A | Dake | Halbert | Hinojosa | Volner | 3:59.82 | Verified |
| 3 | St. James High School | A | Siders | Crocker | Bullock | Shields | 3:58.10 | Unverified: |
| 4 | Steelville High School | A | Diaz | Harris | Killeen | Midyett | 3:35.71 | Verified |
| 5 | Rolla High School | A | Elliott | Harris | Lindsey | Walker | 3:41.03 | Verified |
| 6 | Dixon High School | A | Dalton | Hurst | Dalton | Rester | 3:58.92 | Verified |
| 7 | Vienna High School | A | Auten | Baz | Ewers | Kilmer | 4:08.14 | Verified |