| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Cuba High School | A | Hardcastle | Ijames | Viramontes | Black | 5:09.28 | Verified |
| 2 | St. James High School | A | Deardeuff | Foust | Snyder | Goodin | 4:58.10 | Unverified: |
| 3 | Vienna High School | A | Reeves | Schell | Schulte | Weeks | 4:45.38 | Verified |
| 4 | Rolla High School | A | Inskip | Kriete | Loughridge | Chance | 4:28.35 | Verified |
| 5 | Steelville High School | A | Diaz | Pryor | Pryor | Kreitner | 4:38.13 | Verified |
| 6 | New Haven High School | A | Gerlemann | Luecke | Groner | Strubberg | DNS | Verified |
| 7 | Newburg High School | A | Fite | Logan | Rosa | Twyman | 4:58.10 | Verified |
| 8 | St. Paul Lutheran (Farmington) | A | Blackmon | Baer | Durbin | Mccarty | 5:29.04 | Verified |