| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CAMDEN, Wyatt | FR | Vienna High School | 34.70 | Unverified: |
| 2 | BAILEY, Ethan | SO | Belle High School | 29.95 | Verified |
| 3 | RAMSTEIN, Malachi | SO | Bourbon High School | 29.63 | Verified |
| 4 | RICE, Peyton | SO | New Haven High School | 28.42 | Verified |
| 6 | SEAY, Camrin | SO | Belle High School | 29.81 | Verified |
| 7 | PRATT, Tristan | FR | Newburg High School | 30.48 | Verified |
| 8 | BOATRIGHT, DaVarius | FR | Rolla High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | SHARPENSTEEN, Chase | FR | Crocker High School | 27.36 | Verified |
| 2 | PRADE, Joseph | SO | St. Paul Lutheran (Farmington) | 26.94 | Verified |
| 3 | PERAINO, Lucas | SR | New Haven High School | 26.45 | Verified |
| 4 | FAULKNER, Sean | JR | Crocker High School | 25.54 | Verified |
| 5 | HARDY, Collin | JR | Viburnum High School | 25.82 | Verified |
| 6 | EARLS, Josh | JR | St. James High School | 26.58 | Verified |
| 7 | DECLUE, Keyten | JR | Cuba High School | 27.09 | Verified |
| 8 | JIMENEZ, Sixto | JR | Rolla High School | 28.27 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | REEVES, Austin | SR | Bourbon High School | 24.77 | Verified |
| 2 | HURST, Lane | FR | Dixon High School | 24.68 | Unverified: Vienna High School Black & Gold |
| 3 | HARRIS, Wyatt | SR | Steelville High School | 23.78 | Verified |
| 4 | SNYDER, Kaiden | JR | St. James High School | 22.47 | Verified |
| 5 | JACOBS, Owen | SR | Dixon High School | 23.36 | Verified |
| 6 | VOLNER, Micah | SO | Cuba High School | 24.60 | Verified |
| 7 | MIDYETT, Jacob | JR | Steelville High School | 24.70 | Verified |
| 8 | MINOR, DeVontae | SR | St. Paul Lutheran (Farmington) | 24.79 | Verified |