| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Steelville High School | A | Bailey | Crocker | Rutledge | Moreland | 9:02.30 | Verified |
| 2 | Rolla High School | A | Williams | Clayton | Huckabee | Patmore | 9:02.30 | Verified |
| 3 | Vienna High School | A | Auten | Douglas | Ewers | Kilmer | 9:22.17 | Verified |
| 4 | Dixon High School | A | Dalton | Dalton | McElhaney | Doyle | 9:57.23 | Verified |
| 5 | St. Paul Lutheran (Farmington) | A | Peak | Ziegelmeyer | Carroll | Anderson | 11:02.58 | Verified |