| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | DIAZ, Conner | SR | Steelville High School | 1:59.19 | Verified |
| 3 | CROCKER, Vincent | SO | Steelville High School | 2:16.20 | Verified |
| 4 | WULFF, Troy | FR | Vienna High School | 2:19.00 | Unverified: |
| 5 | SAPPINGTON, Elijah | JR | Bourbon High School | 2:19.44 | Unverified: Vienna High School Black & Gold |
| 6 | ROMO-VAZQUEZ, Jose | JR | New Haven High School | 2:19.85 | Verified |
| 7 | HINOJOSA, Houston | SR | Cuba High School | 2:25.11 | Verified |
| 8 | PEAK, Brett | SR | St. Paul Lutheran (Farmington) | 2:27.79 | Verified |
| 9 | DOUGLAS, Thomas | FR | Vienna High School | 2:29.00 | Unverified: |
| 10 | DALTON, Daxx | JR | Dixon High School | 2:30.45 | Verified |
| 11 | LILLARD, Trevor | FR | Missouri School for the Deaf | 2:31.29 | Verified |
| 12 | FINNEGAN, Barrett | SO | Belle High School | 2:39.97 | Verified |
| 13 | VINEYARD, Gavin | SR | Dixon High School | 2:45.43 | Verified |
| 14 | PRADE, Hudsen | SO | St. Paul Lutheran (Farmington) | 2:56.84 | Verified |
| 15 | VIANA, Jeromie | JR | New Haven High School | 2:57.65 | Verified |
| 16 | DECKER, Alex | JR | Newburg High School | 3:02.37 | Verified |
| 17 | HERR, Kaleb | FR | Cuba High School | NT | Unverified: |
| 18 | WEIGEL, Connor | FR | Belle High School | NT | Unverified: |
| 19 | PATMORE, Jayden | FR | Rolla High School | NT | Unverified: |
| 20 | HUCKABEE, Micah | FR | Rolla High School | NT | Unverified: |