| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | DIAZ, Courtney | SR | Steelville High School | 2:36.77 | Verified |
| 2 | CAQUELIN, Akaela | SO | Newburg High School | 2:37.00 | Verified |
| 3 | CRONIN, Janelle | SO | New Haven High School | 2:45.28 | Verified |
| 4 | WEEKS, Madison | SO | Vienna High School | 2:47.00 | Unverified: |
| 5 | IJAMES, Izzabelle | JR | Cuba High School | 2:47.79 | Verified |
| 6 | COLLINSON, Aleah | JR | Steelville High School | 2:50.34 | Verified |
| 8 | FITE, Brooklyn | FR | Newburg High School | 2:51.42 | Verified |
| 9 | GEBHART, Mia | JR | Bourbon High School | 2:52.48 | Verified |
| 10 | HARDCASTLE, Patricia | FR | Cuba High School | 2:58.86 | Verified |
| 11 | GRUBB, Sydney | JR | New Haven High School | 2:59.21 | Verified |
| 12 | MCCARTY, Taylor | JR | St. Paul Lutheran (Farmington) | 3:05.37 | Verified |
| 13 | BLACKMON, Elissa | JR | St. Paul Lutheran (Farmington) | 3:06.94 | Verified |
| 14 | RANGEL, Samaya | FR | Dixon High School | 3:25.11 | Verified |
| 15 | KLOEPPEL, Ava | SO | Vienna High School | 3:34.64 | Verified |
| 17 | JO WILSON, Andy | FR | Dixon High School | NT | Unverified: |
| 18 | CHANCE, Charlee | FR | Rolla High School | NT | Unverified: |