| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | BEDFORD, Brynley | FR | Rolla High School | NT | Unverified: |
| 3 | EVANS, Norah | JR | Steelville High School | 1:00.65 | Verified |
| 4 | SNYDER, Ava | FR | St. James High School | 58.90 | Unverified: St. James Open |
| 5 | DEARDEUFF, Erika | SR | St. James High School | 59.00 | Unverified: St. James Open |
| 6 | NOVAK, Madeleine | SR | Vienna High School | 1:07.56 | Verified |
| 7 | HIX, Hannah | SO | Bourbon High School | 1:06.74 | Verified |
| 8 | MAUNTEL, Kyra | JR | New Haven High School | 1:08.49 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BARTLE, Raylee | SO | Bourbon High School | 57.50 | Verified |
| 2 | REEVES, Aubrey | SO | Vienna High School | 54.16 | Verified |
| 3 | KREITNER, Abby | JR | Steelville High School | 51.54 | Verified |
| 4 | KECK, Montgomery | JR | Dixon High School | 50.20 | Verified |
| 5 | TWYMAN, Corra | SR | Newburg High School | 50.31 | Verified |
| 6 | WIGGER, Natalie | SO | Viburnum High School | 53.42 | Verified |
| 7 | KLIMA, Carley | FR | St. Paul Lutheran (Farmington) | 55.59 | Verified |
| 8 | CONSTIEN, Sarah | SO | St. Paul Lutheran (Farmington) | 57.69 | Verified |