| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | KINSEY, Kaegan | FR | Belle High School | NT | Unverified: |
| 2 | ROLLINS, Iven | SO | Vienna High School | 1:19.17 | Verified |
| 3 | SEAY, Camrin | SO | Belle High School | 1:09.78 | Verified |
| 4 | ZIMMER, Alex | SO | Vienna High School | 1:07.98 | Verified |
| 5 | SMITH, Jeremy | FR | Cuba High School | 1:08.43 | Verified |
| 6 | TAUSCHER, Marshall | FR | Viburnum High School | 1:18.98 | Verified |
| 7 | LUTTRELL, Mican | FR | Missouri School for the Deaf | 1:26.19 | Verified |
| 8 | WALKER, Zarek | SO | Rolla High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | RESCH, Tristen | FR | Crocker High School | 1:05.70 | Verified |
| 2 | HARDY, Collin | JR | Viburnum High School | 1:03.04 | Verified |
| 3 | MARTIN, Alex | FR | Steelville High School | 1:00.54 | Verified |
| 4 | RESTER, Joshua | FR | Dixon High School | 58.94 | Verified |
| 5 | SIDERS, Brandon | FR | St. James High School | 59.80 | Unverified: St. James Open |
| 6 | MORROW, Evin | FR | Newburg High School | 1:00.83 | Verified |
| 7 | PRADE, Joseph | SO | St. Paul Lutheran (Farmington) | 1:03.63 | Verified |
| 8 | DANIELSON III, Jeffrey | JR | Crocker High School | 1:07.16 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | PIETRASCHKE, Nicholas | JR | Bourbon High School | 58.32 | Verified |
| 2 | DALTON, Waylon | SR | Dixon High School | 57.65 | Verified |
| 3 | MINOR, DeVontae | SR | St. Paul Lutheran (Farmington) | 55.91 | Verified |
| 4 | HARRIS, Wyatt | SR | Steelville High School | 52.61 | Verified |
| 5 | REEVES, Zackary | JR | Bourbon High School | 55.85 | Verified |
| 6 | HINOJOSA, Houston | SR | Cuba High School | 56.92 | Verified |
| 7 | LINDSEY, Trent | SO | Rolla High School | 58.21 | Verified |
| 8 | CROCKER, Logan | SO | St. James High School | 58.90 | Unverified: St. James Open |