| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | FELAN, Addalye | FR | St. James High School | 1:28.85 | Verified |
| 4 | BAER, Anna | SO | St. Paul Lutheran (Farmington) | 1:21.23 | Verified |
| 5 | DAVIS, Isabella | SO | Missouri School for the Deaf | 1:24.37 | Verified |
| 6 | STARNS, Bridgette | FR | Rolla High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | PRATT, Ciarra | JR | Newburg High School | 1:18.95 | Verified |
| 2 | BLACKMON, Elissa | JR | St. Paul Lutheran (Farmington) | 1:18.20 | Verified |
| 3 | WIEBERG, Claudia | JR | Vienna High School | 1:17.00 | Unverified: |
| 4 | IJAMES, Izzabelle | JR | Cuba High School | 1:12.15 | Verified |
| 5 | INSKIP, Samantha | FR | Rolla High School | 1:15.09 | Verified |
| 6 | MURPHY, Morgan | FR | Vienna High School | 1:18.00 | Unverified: |
| 7 | WEBER, Bailey | FR | Cuba High School | 1:18.81 | Verified |
| 8 | BROOKS, Peyton | FR | Steelville High School | 1:21.15 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | LUECKE, Elizabeth | JR | New Haven High School | 1:10.86 | Verified |
| 2 | CLONTS, Cierra | JR | Bourbon High School | 1:09.78 | Verified |
| 3 | ENKE, Marisa | SO | Bourbon High School | 1:06.71 | Verified |
| 4 | MAGNER, Belle | FR | Dixon High School | 1:06.06 | Verified |
| 5 | STRUBBERG, Avery | SO | New Haven High School | 1:06.41 | Verified |
| 6 | CAMPISE, Meghan | SR | Crocker High School | 1:07.37 | Verified |
| 7 | COFFELT, Jazzalyn | FR | Crocker High School | 1:10.11 | Verified |
| 8 | CONAWAY, Jorja | JR | Viburnum High School | 1:10.88 | Verified |