| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Dixon High School | A | Doyle | O'neal | Dalton | Rester | 53.06 | Verified |
| 4 | Crocker High School | A | Bjornstead | Faulkner | Goldsmith | Smith | 50.76 | Verified |
| 5 | Belle High School | A | Bailey | Gromowski | Seay | 51.70 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Vienna High School | A | Baz | Hamill | Kilmer | Carnes | 49.98 | Verified |
| 3 | Cuba High School | A | Dake | Halbert | Hinojosa | Volner | 47.57 | Verified |
| 4 | Rolla High School | A | Plank | Harris | Wassilak | Gibson | 45.57 | Verified |
| 5 | St. James High School | A | Spurgeon | Earls | Noakes | Bell | 47.38 | Verified |
| 6 | Steelville High School | A | Killeen | Brown | Midyett | Settles | 48.46 | Verified |
| 7 | St. Paul Lutheran (Farmington) | A | Folk | Anderson | Lindsey | Garner | 50.08 | Verified |