| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Cuba High School | B | NT | |||||
| 3 | Missouri School for the Deaf | A | Davis | Freeman | Hull | Smith | 1:07.77 | Verified |
| 4 | Cuba High School | A | Lunsford | Martellaro | Moreland | Dunham | 59.45 | Verified |
| 5 | St. Paul Lutheran (Farmington) | A | Eller | Durbin | Govero | Baer | 1:00.22 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Vienna High School | A | Schulte | Rosentreter | Schell | Novak | 58.41 | Verified |
| 2 | New Haven High School | A | Faris | GUEHNE | Peraino | Groner | 57.02 | Verified |
| 3 | St. James High School | A | Jones | Campbell | Terrill | Hughes | 56.56 | Unverified: Union Invitational |
| 4 | Rolla High School | A | Alamaldein | Brooks | Henry | Kriete | 55.11 | Verified |
| 5 | Steelville High School | A | Eilert | Pryor | Pryor | Kreitner | 56.18 | Verified |
| 7 | Belle High School | A | Rehmert | Bailey | Koch | Naber | 57.86 | Verified |