| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | DIAZ, Conner | SR | Steelville High School | 4:28.98 | Verified |
| 2 | PAYNE, Brett | FR | Bourbon High School | 4:45.76 | Verified |
| 3 | AUTEN, Cooper | SO | Vienna High School | 5:05.25 | Verified |
| 4 | SHIELDS, Luke | JR | St. James High School | 5:12.96 | Verified |
| 5 | EWERS, Brayden | JR | Vienna High School | 5:15.57 | Verified |
| 6 | BAILEY, Curtis | JR | Steelville High School | 5:25.01 | Verified |
| 7 | MCELHANEY, Kaleb | SO | Dixon High School | 5:28.00 | Verified |
| 8 | DANIELSON III, Jeffrey | JR | Crocker High School | 5:33.96 | Verified |
| 9 | MOORE, Seth | JR | Dixon High School | 5:35.23 | Verified |
| 10 | ZIEGELMEYER, Wyatt | SO | St. Paul Lutheran (Farmington) | 5:37.03 | Verified |
| 11 | BROOKS, Gabriel | JR | Viburnum High School | 5:38.08 | Verified |
| 12 | WILLIAMS, Scott | FR | Rolla High School | 5:44.42 | Verified |
| 13 | FINNEGAN, Barrett | SO | Belle High School | 6:07.90 | Verified |
| 14 | RODRIGUEZ, Carlos | JR | Rolla High School | 6:19.42 | Verified |
| 15 | CARROLL, Jacob | SR | St. Paul Lutheran (Farmington) | 6:20.15 | Verified |
| 16 | HOEHNE, Adrien | JR | Belle High School | 6:25.98 | Verified |
| 17 | DECKER, Alex | JR | Newburg High School | 6:36.37 | Verified |