| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | LEWIS, Sariah | SO | Jennings High School | NT | Unverified: |
| 2 | STRINGFELLOW, Eryana | JR | Lutheran North High School | NT | Unverified: |
| 3 | PITTMAN, Cierra | SO | Clayton High School | NT | Unverified: |
| 4 | COLEMAN, Angel | SO | Jennings High School | 36.13 | Verified |
| 5 | ROLLEY, Vonyae | SO | Jennings High School | NT | Unverified: |
| 6 | MCFEE, Lashera | FR | Affton High School | NT | Unverified: |
| 7 | MANUEL, Jaila | JR | Affton High School | NT | Unverified: |
| 8 | ZAIDMAN, Naomi | FR | Clayton High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | ROTH, Samantha | JR | Mehlville High School | 32.21 | Verified |
| 2 | HUGHES, PAIGE | SO | Oakville High School | 29.22 | Verified |
| 3 | JONAS, MADYSEN | JR | Oakville High School | 28.84 | Verified |
| 4 | SANDERS, Mekhila | JR | Clayton High School | 26.01 | Verified |
| 5 | GLADNEY, Diamond | SR | Lutheran North High School | 28.34 | Verified |
| 6 | MUELLER, Jenna | SR | Oakville High School | 28.84 | Verified |
| 7 | NASUFOVIC, Adna | JR | Mehlville High School | 29.77 | Verified |
| 8 | TREVISANO, TAYLOR | SR | Mehlville High School | 32.50 | Verified |