| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CLARK, Michael | FR | Lutheran North High School | NT | Unverified: |
| 2 | AVERBECK, Peyton | FR | Affton High School | NT | Unverified: |
| 3 | PALMER, Caleb | SR | Lutheran North High School | NT | Unverified: |
| 4 | MORALES, Kevin | FR | Affton High School | NT | Unverified: |
| 5 | HEFLIN, Keaton | SO | Oakville High School | NT | Unverified: |
| 6 | SUN, Samuel | JR | Clayton High School | NT | Unverified: |
| 7 | HUNT, Hayden | SR | Vianney High School | NT | Unverified: |
| 8 | ZERIC, Dino | JR | Affton High School | NT | Unverified: |
| 9 | KALACHEK, Jacob | FR | Clayton High School | NT | Unverified: |
| 10 | KARANDJEFF, John | FR | Clayton High School | NT | Unverified: |
| 11 | FUNEZ, Joel | FR | Oakville High School | NT | Unverified: |
| 12 | WEISS, William | FR | Affton High School | NT | Unverified: |
| 13 | KELLER, Graham | JR | Clayton High School | NT | Unverified: |
| 14 | CHOU, Isaac | SO | Vianney High School | NT | Unverified: |
| 15 | THIERAUF, Lucas | JR | Vianney High School | NT | Unverified: |
| 16 | MENDENHALL, Logan | FR | Affton High School | NT | Unverified: |
| 17 | OETTING, Carter | FR | Oakville High School | NT | Unverified: |
| 18 | AL-KENANI, Ali | FR | Oakville High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | JOHNSON, Sylvester | JR | Lutheran North High School | 2:03.18 | Verified |
| 2 | LOUVIERRE, KENNETH | SR | Oakville High School | 2:13.62 | Verified |
| 3 | GOLD, JASON | JR | Oakville High School | 2:13.77 | Verified |
| 4 | LOVELACE, Josiah | JR | Affton High School | 2:16.42 | Verified |
| 5 | ROCHE, Andrew | JR | Vianney High School | 2:24.85 | Verified |
| 6 | MOONEY, Tyler | SO | Affton High School | 2:25.95 | Verified |
| 7 | BENNETT, Brandon | JR | Affton High School | 2:30.64 | Verified |
| 8 | BARNES, Marrione | FR | Lutheran North High School | 2:34.39 | Verified |
| 9 | MOEHLENBROCK, Bailey | JR | Affton High School | 2:34.62 | Verified |
| 10 | LANG, Connor | SO | Affton High School | 2:36.11 | Verified |
| 11 | SANER, Bora | JR | Clayton High School | 2:47.84 | Verified |
| 12 | OMEROVIC, Emrah | SR | Affton High School | 2:49.65 | Verified |
| 13 | DODOLEWA, Simisola | JR | Lutheran North High School | 2:53.39 | Verified |
| 14 | DULLOVI, Avni | SO | Affton High School | 2:58.87 | Verified |
| 15 | KELLY, Dylon | SO | Affton High School | 3:02.92 | Verified |
| 16 | SKAMPO, Eldin | SO | Affton High School | 3:09.86 | Verified |
| 17 | STREET, Corey | SO | Affton High School | 3:17.99 | Verified |
| 18 | VAHEY, Camden | FR | Vianney High School | NT | Unverified: |