| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | JONES, Eden | SR | Lutheran North High School | NT | Unverified: |
| 4 | CELLA, Emelia | SO | Affton High School | NT | Unverified: |
| 5 | LERENA, Lucia | SO | Clayton High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HOOTEN, Janna | SO | Jennings High School | NT | Unverified: |
| 2 | DANIEL, Eliza | SO | Affton High School | 1:04.36 | Verified |
| 3 | CROSS, Cesarine | SO | Clayton High School | 54.78 | Verified |
| 4 | O'SHEA, Jenna | SR | Mehlville High School | 51.13 | Verified |
| 5 | ABDIJANOVIC, Anela | JR | Mehlville High School | 52.52 | Verified |
| 6 | ISLAM, SABRINA | JR | Oakville High School | 57.47 | Verified |
| 7 | JEANS, Madeline | SO | Clayton High School | 1:05.97 | Verified |
| 8 | LEWIS, Sariah | SO | Jennings High School | NT | Unverified: |