| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | LE, Kyle | JR | Oakville High School | 5:04.56 | Verified |
| 2 | SANER, Bora | JR | Clayton High School | 5:44.20 | Verified |
| 3 | MOONEY, Tyler | SO | Affton High School | 5:54.22 | Verified |
| 4 | EARLL, Mason | SO | Affton High School | 5:55.88 | Verified |
| 5 | LANG, Connor | SO | Affton High School | 6:16.85 | Verified |
| 6 | KELLY, Dylon | SO | Affton High School | 6:24.00 | Verified |
| 7 | DULLOVI, Avni | SO | Affton High School | 6:32.65 | Verified |
| 8 | SKAMPO, Eldin | SO | Affton High School | 6:41.83 | Verified |
| 9 | STREET, Corey | SO | Affton High School | 7:20.23 | Verified |
| 10 | DANFORTH, Vincent | FR | Vianney High School | NT | Unverified: |
| 11 | HEFLIN, Keaton | SO | Oakville High School | NT | Unverified: |
| 12 | DOBBINS, NOAH | SO | Oakville High School | NT | Unverified: |
| 13 | AL-KENANI, Ali | FR | Oakville High School | NT | Unverified: |
| 14 | MURPHY, Bryan | SO | Clayton High School | NT | Unverified: |
| 15 | AVERBECK, Peyton | FR | Affton High School | NT | Unverified: |
| 16 | OMEROVIC, Emrah | SR | Affton High School | NT | Unverified: |
| 17 | DUFFEY, Conner | FR | Vianney High School | NT | Unverified: |
| 18 | WEISS, William | FR | Affton High School | NT | Unverified: |
| 19 | KREISZ, Kolton | SO | Oakville High School | NT | Unverified: |
| 20 | ZERIC, Dino | JR | Affton High School | NT | Unverified: |
| 21 | BOGAN, Jeremiah | SR | Lutheran North High School | NT | Unverified: |
| 22 | SCHELP, Henry | FR | Oakville High School | NT | Unverified: |
| 23 | LOPORTO, Logan | FR | Vianney High School | NT | Unverified: |
| 24 | MENDENHALL, Logan | FR | Affton High School | NT | Unverified: |
| 25 | FIELDS, William | FR | Clayton High School | NT | Unverified: |
| 26 | FUNEZ, Joel | FR | Oakville High School | NT | Unverified: |
| 27 | OETTING, Carter | FR | Oakville High School | NT | Unverified: |
| 28 | SOKOLIK, Alexander | FR | Oakville High School | NT | Unverified: |