| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Affton High School | A | Daughtry | Cousins | Smith | Wilson | 1:56.88 | Verified |
| 4 | Clayton High School | A | Musiek | Sanders | Ramirez Taylor | Miller | 1:50.72 | Verified |
| 5 | Francis Howell High School | A | Nguyen | Sidwell | Lewis | Lewis | 1:51.58 | Verified |
| 6 | Clayton High School | B | Kabargina | Wolpert | Zaidman | Zimmerman | DQ | Unverified: 11th Annual Bob G. Brunette Invi |