| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Nerinx Hall High School | A | McClure | Clyne | Andres | Banks | 5:00.00 | Unverified: |
| 4 | Francis Howell High School | A | Reichart | Moran | Ward | Poinsett | NT | Verified |
| 5 | Affton High School | A | Cousins | Daughtry | Olesia | Wilson | 4:40.82 | Verified |
| 6 | Clayton High School | A | Musiek | Ramirez Taylor | Wolpert | Erdmann | NT | Unverified: |