| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | FOLUKE, Amari | SR | Metro High School | NT | Unverified: |
| 3 | WHITE, Gavin | FR | Metro High School | 28.55 | Verified |
| 4 | FULLER, Amari | FR | Affton High School | 27.10 | Verified |
| 5 | IRVING, Alexander | FR | Clayton High School | 27.24 | Verified |
| 6 | DAVIS, Nathanael | FR | Affton High School | 28.58 | Verified |
| 7 | ANDERSON-JENNINGS, Lorenz | SR | Clayton High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CARTER, Devin | JR | Metro High School | 26.12 | Verified |
| 2 | HORTHORN, Thajay | FR | Jennings High School | 25.05 | Verified |
| 3 | CASON, Cameron | SO | Francis Howell High School | 24.28 | Verified |
| 4 | CHATMAN, Joshua | SR | Clayton High School | 23.90 | Verified |
| 5 | BALLARD, Jaylen | FR | Francis Howell High School | 23.97 | Verified |
| 6 | GARBARINI, Hunter | JR | Francis Howell High School | 24.44 | Verified |
| 7 | MARTIN, Merrimon | FR | Jennings High School | 25.25 | Verified |
| 8 | ALLEN, Zachary | FR | Affton High School | 26.26 | Verified |