| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Metro High School | A | Baum-Scholle | Gonzalez | Keoshkerian | Murch | 9:26.34 | Verified |
| 2 | Clayton High School | A | Murphy | Keller | Saner | Sun | 9:29.79 | Verified |
| 3 | Affton High School | A | Lang | Mooney | Earll | Kelly | 9:30.93 | Verified |
| 4 | Affton High School | B | Weiss | Street | 9:30.93 | Verified | ||
| 5 | Affton High School | C | NT | Verified |