| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | WILLETT, Angelica | SR | Francis Howell High School | NT | |
| 2 | ZIMMERMAN, Riley | SO | Clayton High School | NT | Unverified: |
| 3 | KABARGINA, Margaret | FR | Clayton High School | 32.21 | Verified |
| 4 | WATERS, Kirsten | SR | Francis Howell High School | 31.64 | Verified |
| 5 | IVORY, Alexandria | FR | Nerinx Hall High School | 31.83 | Verified |
| 6 | O'GORMAN, Edith | SO | Nerinx Hall High School | 32.62 | Verified |
| 7 | MCFEE, Lashera | FR | Affton High School | 33.96 | Verified |
| 8 | STAGE, Sophia | SO | Nerinx Hall High School | 35.60 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | LEWIS, Sariah | SO | Jennings High School | 31.33 | Verified |
| 2 | NIEDERS, Olivia | SO | Francis Howell High School | 30.94 | Verified |
| 3 | GONZALES, Amari | JR | Francis Howell High School | 28.94 | Verified |
| 4 | SANDERS, Mekhila | JR | Clayton High School | 26.01 | Verified |
| 5 | MACON-FORD, Leah | JR | Metro High School | 27.91 | Verified |
| 6 | GRIFFITH, Naomi | SR | Metro High School | 29.63 | Verified |
| 7 | CALLOWAY, Nadia | SO | Metro High School | 30.99 | Verified |
| 8 | SMITH, Aymerie | JR | Affton High School | NT |