| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | TRANKLER, Nicholas | FR | Francis Howell High School | 2:45.22 | Verified |
| 2 | DEES, Jhakovi | SO | Francis Howell High School | 2:45.60 | Verified |
| 3 | KIMBLE, Christian | JR | Francis Howell High School | 2:48.95 | Verified |
| 4 | OMEROVIC, Emrah | SR | Affton High School | 2:49.65 | Verified |
| 5 | KELLY, Dylon | SO | Affton High School | 2:52.15 | Verified |
| 6 | LONG, Rowan | SO | Metro High School | 2:55.34 | Verified |
| 7 | MENDENHALL, Logan | FR | Affton High School | 2:56.60 | Verified |
| 8 | TAMME, Colton | FR | Francis Howell High School | 2:57.35 | Verified |
| 9 | DULLOVI, Avni | SO | Affton High School | 2:58.87 | Verified |
| 10 | MORALES, Kevin | FR | Affton High School | 2:59.52 | Verified |
| 11 | SKAMPO, Eldin | SO | Affton High School | 3:09.86 | Verified |
| 12 | MILLS, Owen | SO | Francis Howell High School | NT | Unverified: |
| 13 | PANUS, Aiden | SO | Francis Howell High School | NT | Unverified: |
| 14 | ZEPEDA, Salvador | SO | Francis Howell High School | NT | Unverified: |
| 15 | COHEN, Wade | FR | Francis Howell High School | NT | Unverified: |
| 16 | WALKER, Nigel | FR | Francis Howell High School | NT | Unverified: |
| 17 | RADER, Drew | SO | Francis Howell High School | NT | Unverified: |
| 18 | BETHEL, Weston | FR | Affton High School | NT | Unverified: |
| 19 | LINK, Sydney | SO | Francis Howell High School | NT | Unverified: |
| 20 | LOGAN, Kaden | SO | Francis Howell High School | NT | Unverified: |
| 21 | PETTI, Hayden | FR | Affton High School | NT | Unverified: |
| 22 | AVERBECK, Peyton | FR | Affton High School | NT | Unverified: |
| 23 | COCKRILL, Cameron | SO | Francis Howell High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | THOMA, Sam | SR | Francis Howell High School | 2:12.57 | Verified |
| 2 | KOENEKER, Alexander | SO | Francis Howell High School | 2:13.16 | Verified |
| 3 | GHEORGHIAN, Kaiden | JR | Ladue Horton Watkins High School | 2:13.79 | Verified |
| 4 | NORDGAARDEN, Chase | SR | Francis Howell High School | 2:13.82 | Verified |
| 5 | HOETTE, Charles | SR | Clayton High School | 2:16.97 | Verified |
| 6 | CHUC, Ethan | SO | Francis Howell High School | 2:18.89 | Verified |
| 7 | CADE, Keteyian | JR | Metro High School | 2:20.24 | Verified |
| 8 | CUSTIS, Andrew | SR | Metro High School | 2:21.84 | Verified |
| 9 | ESTRADA, Javier | FR | Metro High School | 2:22.75 | Verified |
| 10 | FRANKE, Luke | FR | Francis Howell High School | 2:23.41 | Verified |
| 11 | GALBURT- RUIZ, Martin | FR | Metro High School | 2:23.70 | Verified |
| 12 | UMSTEAD, Nylan | SR | Francis Howell High School | 2:24.61 | Verified |
| 13 | BAKER, Luke | JR | Clayton High School | 2:25.66 | Verified |
| 14 | MOONEY, Tyler | SO | Affton High School | 2:25.95 | Verified |
| 15 | ZOU, Dante | FR | Clayton High School | 2:26.71 | Verified |
| 16 | KIRCHHOFF, Nathan | FR | Francis Howell High School | 2:33.00 | Verified |
| 17 | KELLER, Graham | JR | Clayton High School | 2:33.19 | Verified |
| 18 | VAN ANNE, Dax | FR | Francis Howell High School | 2:33.98 | Verified |
| 19 | LANG, Connor | SO | Affton High School | 2:34.57 | Verified |
| 20 | MOEHLENBROCK, Bailey | JR | Affton High School | 2:34.62 | Verified |
| 21 | RILEY, Colin | FR | Francis Howell High School | 2:35.47 | Verified |
| 22 | SANER, Bora | JR | Clayton High School | 2:41.57 | Verified |
| 23 | ZERIC, Dino | JR | Affton High School | 2:41.66 | Verified |
| 24 | WEISS, William | FR | Affton High School | 2:42.79 | Verified |