| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | GRIFFEY, Ilse | JR | Clayton High School | NT | Unverified: |
| 4 | CARAFIOL, Luci | FR | Francis Howell High School | NT | Unverified: |
| 5 | BERNSTEIN, Sage | JR | Clayton High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | JEANS, Madeline | SO | Clayton High School | 1:05.66 | Verified |
| 2 | CARAFIOL, Anna | SO | Francis Howell High School | 1:02.98 | Verified |
| 3 | FALLER, Ashley | SO | Francis Howell High School | 1:00.58 | Verified |
| 4 | TUCKER, Mallory | SO | Francis Howell High School | 59.87 | Verified |
| 5 | TRODUS, Lauren | FR | Clayton High School | 1:00.21 | Verified |
| 6 | LERENA, Lucia | SO | Clayton High School | 1:01.58 | Verified |
| 7 | DANIEL, Eliza | SO | Affton High School | 1:04.36 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | WAGSTAFF, Elise | SO | Affton High School | 59.15 | Verified |
| 2 | SWEETEN, Addison | FR | Francis Howell High School | 57.39 | Verified |
| 3 | TRANKLER, Isabella | SO | Francis Howell High School | 54.27 | Verified |
| 4 | REICHART, Brigid | JR | Francis Howell High School | 47.08 | Verified |
| 5 | POSTAL, Ella | SR | Francis Howell High School | 52.03 | Verified |
| 6 | CROSS, Cesarine | SO | Clayton High School | 54.78 | Verified |
| 7 | CHRISTENSEN, Madison | SO | Francis Howell High School | 58.47 | Verified |
| 8 | MCCLURE, Clare | FR | Nerinx Hall High School | 59.50 | Unverified: |